You don't need to write code  ( it may be varies according to your requirment)
u can directly link ur Excel into Access
Pls try this Open ur DB(Access)

Goto File>Get Entrenal data>Link table
In this way whenever you make any changes into Excel It will reflect to your
Access DB

Other way, Excel gives you a facility to creat you own table in Excel.
Please read the help  "Name cells in a workbook"
Now you can use ADO to load the data  into Access DB

I want store data  from to excel  sheet,at a time check the DB , if db values
and textbox values are same not stroe in the excel sheet,(check db values after
that add excel sheet),

If txtbox1 values store only one time but txtbox2 values store more then 100
time,(both excel sheet  and DB)