Yes. You can write your own TraceListener-derived class, and direct all output through it. Here's a simple example, which derives from TextWriterTraceListener (and therefore has in-built support for writing to files, as shown above) and adds timing information and the thread ID for each trace line:

class MyListener : TextWriterTraceListener
{
public MyListener( Stream s ) : base(s)
{
}

public override void WriteLine( string s )
{
Writer.WriteLine( "{0:D8} [{1:D4}] {2}",
Environment.TickCount - m_startTickCount,
AppDomain.GetCurrentThreadId(),
s );
}

protected int m_startTickCount = Environment.TickCount;
}
(Note that this implementation is not complete - the TraceListener.Write method is not overridden for example.)

The beauty of this approach is that when an instance of MyListener is added to the Trace.Listeners collection, all calls to Trace.WriteLine() go through MyListener, including calls made by referenced assemblies that know nothing about the MyListener class.